Retroviral recombination is temperature dependent
نویسندگان
چکیده
منابع مشابه
Retroviral recombination during reverse transcription.
After mixed infection, up to half of related retroviruses are recombinants. During infection, retroviral RNA genomes are first converted to complementary DNA (cDNA) and then to double-stranded DNA. Thus recombination could occur during reverse transcription, by RNA template switching, or after reverse transcription, by breakage and reunion of DNA. It has not been possible to distinguish between...
متن کاملModification for the Mechanism of Retroviral Recombination
Figure 1: The original minus strand exchange model. (A) The retroviral virion contains two essentially identical RNA molecules. (B) An extended singlestranded DNA molecule is left tailing behind. This single-stranded DNA tail is free to form a hybrid duplex with another RNA molecule. (C) The original template will be displaced by branch migration of the second RNA. This second RNA molecule will...
متن کاملRetroviral recombination: review of genetic analyses.
Retroviruses package two copies of genomic RNA into one virion. One of the essential steps of replication is reverse transcription, in which the virally encoded enzyme reverse transcriptase (RT) uses the packaged RNA as a template to synthesize viral DNA. Because two copies of RNA are present in one virion, it is possible for RT to switch from one copy of the viral RNA to the other copy during ...
متن کاملTemperature-dependent recombination in polymer composite light-emitting diodes
We study the temperature dependence of the current–voltage and radiance–voltage curves in double-carrier injected polymer light-emitting devices comprised of poly~2-methoxy,5~28-ethyl-hexoxy!– p-phenylene vinylene) ~MEH–PPV! and MEH–PPV/SiO2 as the active layer. The quantum efficiency increases significantly as the temperature is decreased in agreement with an increase in the recombination effi...
متن کاملThe recombination rate is not increased when retroviral RNA is missing an encapsidation sequence.
Retroviruses, as a result of the presence of two identical RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination occurs, although the rate is only 0.1% of the rate of homologous recombination. We developed a protocol to study transduction of cellular proto-oncogenes in a single cycle of retrovirus repl...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2001
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-82-6-1359